Part 6 - Pseudouridine; links re microRNA, and SV40 for lay readers or the discovery in CoV jabs; and What is cancer?

Links to work by Inugo Montoya, Jennifer Brown, and Kevin McKernan.

CoV and cancer is a huge topic and others with more experience than I in the topic, have written about parts of the story. Polyphenols effect on microRNA is what I have spent some time reading about.

• This post is a continuation of a series regarding CoV injections and cancer, and microRNA’s regulatory control over normal mRNA, Part 5. Annoyed Citizen Interview with Kevin McCairn & a preprint re jab effects on innate and adaptive immune system (substack.com)

Unfortunately the CoV jab mRNA is 100% psudeuridylated and that makes it difficult for our body to break down normally. Ccan plant polyphenols help? Maybe. Early treatment with varied phytonutrients or plant extracts does seem to help. The post wanders a bit but looks at those questions a little closer, using links shared by Annoyed Citizen or others I have found.

Inugo Montoyo also mentions the idea of plant polyphenols as a possible solution for the spike protein issue of fragmented lengths doing who knows what damage. The spike is bad, the smaller bits it breaks down into also seem bad and a misfolding risk. Might they also be affecting regulation of other mRNA too? We don’t really know.

• More about the proteins involved in making microRNA, ‘Inugo Research - miRNA’, - Inugo Montoyo (*an anonymous name) is concerned that the chopped up chimeric spike fragments may be cancer promoting and they also have been found to be a misfolding protein risk.

Inugo Research - miRNA

That many scientists have to use anonymity to write about CoV spike issues is a sad statement about our censored times.

Many researchers in certain fields became suddenly dead … prior to the CoV sudden death era. GcMAF - vitamin D Binding protein and autism was one topic and HIV/AIDS was another topic with many suddenly dead researchers.

Just sayin’

Running a meth lab is supposed to be the dangerous industry, not academia.

Pseudouridine replacing uridine is a big problem with the mRNA jabs. Our body cannot regulate it with our microRNA. Can plant polyphenols affect it though?

I am leaning towards thinking positive - early treatment with many different phytonutrients and low budget medications have worked - suggesting that treatment can work when started early enough to prevent full hyperinflammation. Once that is happening things are harder to reset, but nutrient and phytonutrient treatment still seems to help, when provided.

The plant wants to survive the cold snap, or the drought or heatwave, and it has tools that can reset things under varied conditions. That can help us too, but not if we don't eat the whole food substances. Over cooked, over processed, thrown away in the cooking water, try to see those nutrients as part of the food value to preserve and use.

Addition: more about therapeutics by Doorless Carp:

Selected therapeutics and their interactions with lncRNAs

• Dr. Jennifer Brown has a series of three posts for lay readers about the SV40 sequence in the DNA plasmids that can be a cancer risk. This is the first in the series:

What is the Simian 40 (SV40) virus?

The three posts are not lengthy and do build on each other, reading in order helps. In a nutshell, SV40 is a monkey virus that seems to have become incorporated into polio vaccines that were developed in rhesus monkeys. That polio vaccine was eventually stopped for use, but the SV40 virus seems to have gotten into enough humans that it spreads from human to human too - which equals endemic, like a cold or flu virus. Except the SV40 virus is highly associated with cancer promotion. (First post) Keven McKernan and a Japanese team or researcher found the SV40 sequence in DNA plasmids in the CoV injections - there is NO REASON for it to be in a coronavirus vaccine. It is a known cancer promoting risk factor. (Second post)

• Kevin McKernan, PhD, has a post about the SV40 sequence, he writes as Anandamide on Substack, and it is a little science heavy (or a lot): Sequencing the Pfizer monovalent mRNA vaccines also reveals dual copy 72-bp SV40 Promoter, (substack.com).

More about Pseudouridine modifications in RNA - a tiny amount is present normally, 0.2-0.6% overall.

"Of the 166 known RNA nucleoside modifications, pseudouridine (Ψ) is the most abundant post-transcriptional modification and was the first to be discovered [1,2]. The isomerization of 1-ribosyluracil (uridine) to 5-ribosyluracil provides an additional hydrogen bond donor on the uracil base that contributes to the stabilization of base-stacking and RNA structures [3].

[»] Known as the fifth ribonucleoside, Ψ is widely distributed in almost all RNA-containing species, accounting for over 1% of total nucleosides and over 7% of all uridines in ribosomal RNA (rRNA) fractions.

[Benefits of natural pseudouridiylation of RNA]: Ψ has been shown to improve the binding of transfer RNA (tRNA) to ribosomes and the translational fidelity of rRNAs [4,5]. It is also involved in the regulation of splicing of pre-mRNAs in small nuclear splice RNAs (snRNAs) as well as in the decoding and stabilization of tRNAs (overview in [6]). Recently, with the advent of massive parallel transcriptome sequencing, hundreds of naturally modified Ψ-sites in mRNA in yeast and human cells have been discovered [7,8,9].

[»] In particular, about 0.2-0.6% of uridines in mammalian mRNA are pseudouridylated [10].

The pseudouridylation profile in mRNA was dynamically and stress-specifically regulated [7,8,10]. Although the biological consequences of pseudouridylation of mRNA are not yet known, it is postulated that the irreversible changes in the pseudouridylation profile of the transcriptome could lead to functional changes, including protein recoding, reduced translation efficiency, and altered transcript structure [11]."

[…] “Using our optimized HPLC assay, we found that 1.20% and 1.94% of all ribonucleosides in nuclear-enriched RNA and small non-coding RNA pools from the HEK293 cell line, and 1.77% and 0.98% of ribonucleosides in 18S and 28S rRNA isolated from the HeLa cell line, were pseudouridylated.” (Xu, Gu, Thumati, Wong, 2017)

Annoyed Citizen points out that the CoV jab mRNA had 100% substitution of pseudouridine, (Nance, Meier, 2021):

“"In an early study (for which the Sigma-Aldrich catalogue is very grateful), pseudouridine was isolated from 20 liters of urine donated by patients with leukemia, polycythemia, or gout, converted to radiolabeled triphosphate by a mixed chemoenzymatic approach, and found to replace uridine in RNA during in vitro transcription when UTP is omitted. Early studies on T7 RNA polymerase found that it also allows modified NTPs that do not alter base pairing, and this strategy has since been applied to many different bases.

[»] A disadvantage of this enzymatic approach is that it homogeneously replaces the natural nucleobase with a non-natural residue; in the case of BNT162b2, any uridine residue in the mRNA is replaced by m1Ψ."” (Nance, Meier, 2021)

The benefits of pseudouridylation for the pharmaceutical company - is reduced recognition and breakdown by our innate immune system:

“Post-transcriptional modifications naturally occurring in the mRNA nucleotides sequence prevent its immune detection, which, in contrast, is commonly triggered by pathological or invading mRNA. Recognition from innate immune sensors can be avoided by incorporating chemically modified nucleosides, such as ψ [53] and m1ψ [50] (present in transfer and ribosomal RNAs) to prevent activation of TLR7, TLR8, and other innate immune sensors [54], hence reducing type I interferon production [51].” (Granados-Riveron, Aquino-Jarquin, 2021)

Annoyed Citizen repeats this part of the previous excerpt about the natural rate of psuedouridylation – about 0.2-0.6% in mammalian mRNA, 7% in ribosomal RNA – the passage is worth repeating because it “is so frightening” – biological consequences of increased pseudouidylation are unknown but might include functional changes such as protein recoding, less efficient protein translation - more errors occurring in the amino acids being strung together, and alterations in structure of the protein being produced - a different shape will be made (I think):

"The pseudouridylation profile in mRNA was dynamically and stress-specific regulated [7,8,10]. Although the biological consequences of pseudouridylation of mRNA are not yet known, it is postulated that the irreversible changes in the pseudouridylation profile of the transcriptome could lead to functional changes, including protein recoding, reduced translation efficiency, and altered transcript structure [11]." (Xu, Gu, Thumati, Wong, 2017)

Something else, link from a Tweet by NarfGB - Annoyed Citizen’s current Twitter account:

“However, it appears that the complexity of RNA-based regulation increases with the complexity of the organism, creating additional regulatory circuits, the scope of which is only now being revealed. It is becoming apparent that previously unappreciated features, such as disordered structural regions in proteins or non-coding regions in DNA leading to higher plasticity and pliability in RNA–protein complexes, are in fact essential for complex, precise and fine-tuned regulation.”

‘RNA–protein interactions: disorder, moonlighting and junk contribute to eukaryotic complexity,’ (Balcerak, et al., 2019)

The more complex an organism is, the more the noncoding sections of DNA may be essential for RNA based regulation. “Disordered structural regions in proteins or non-coding regions in DNA” may help RNA-protein complexes to form in modified ways - “plasticity and pliability”.

“Recent efforts to identify new RNA-binding proteins (RBPs) in a screening technique called RNA interactome capture revealed that there might be about 1500–1900 RBPs in human cells [2], many more than previously estimated. Many of these proteins do not possess a canonical RNA-binding domain (RBD) and instead harbour an intrinsically disordered region (IDR), with RNA-binding potential. Structural disorder allows for more flexible and dynamic RNA binding, which contributes to the precision of the cellular response to stress and signalling. These new findings suggest that there is a whole new avenue of research to explore and that the role of RBPs in generating complexity may have been underestimated.” (Balcerak, et al., 2019)

The complexity of life is often underestimated in my opinion - as a prenatally focused nutritionist. How do women grow a baby from a few additional food calories per day? (Extra nutrients are needed more than extra calories, especially in the first trimester, then it is about one extra largish snack/smallish meal worth of extra protein, healthy fats and carbs.

Photo by Jan Canty on Unsplash

With the issue of 100% pseudouridylation disrupting precise microRNA control of the pseudouridylated CoV mRNA - the information that disordered or non-coding sections in DNA may have a more generalized ability or more flexible ability to regulate RNA-protein complexes is somewhat hopeful (to many unknowns to me at this time to say more than it seems hopeful). Maybe like the innate immune system, nature has provided more flexible ways to cope with unusual RNA. Plant phytonutrients and other nutrients have shown consistently to help CoV issues, particularly if given early.

Addition - Christie Laura Grace just posted a lengthy discussion that includes RNA binding proteins and one that is linked to cancer and affected by the SV40 sequence. She puts our puzzle pieces together, speculatively. More research is needed. It had largely been banned or discouraged.

Turbo Cancer: Pfizer mRNA Vaccines, DNA Contamination, the SV40 Promoter, and DEAD-Box Proteins

In my own journey through chronic illness and discovery of gene differences that set me up for a tougher time (in some areas), I grew to appreciate redundancy. How can life work? It is so complex!

» Redundancy I think. Nature loves a good design and nature loves workarounds. There often seems to be many ways to do something in the body and one dysfunction may be offset by an adaptation. The plant polyphenols in the large microRNA study reset microRNA changes that had occurred in response to a genetic difference in the mouse population being used. We have to eat the plant polyphenols though, or they can’t help us.

• This post: microRNA are the real regulators of gene transcription. (substack.com)

• This great paper has huge supplementary data Tables which could probably be used to write additional papers. It is Open Source. ‘miRNA as molecular target of polyphenols underlying their biological effects’. (Milenkovic, Jude, Morand, 2013)

We do know that lack of early treatment does not help.

Dietitian warning - what I also know is that leaving a patient with nothing by mouth or minimal food for a week or more and then sticking them on a full-feed drip tube in the ICU will set up metabolic distress, add venting and sedating and organ failure is pretty likely. What I observed of ‘standard hospital care’ was that patients are left far too long with little food and then overdripped with saline or tube feeding bags. Skin health is neglected, allowed to dry and chap. It seems more like really expensive neglect dedicated to monitoring machines and the patient is incidental - but so appreciated and loved by the caring, caring staff. They seem to mean well but there are reports that patients who were un-CoV-jabbed were really mistreated compared to the jabbed who ended up hospitalized for CoV.

If hospital staff are simply monitoring ferritin levels as a “sign of inflammation or infection” then get out of there as quickly as possible. They don’t understand hyperinflammation and are simply monitoring people into greater illness.

Someone on Twitter claimed my Tweet about the real cause of cancer was encouraging people not to get cancer ‘treatment’. My Tweet hadn’t gone that far at all - but - if all they do is monitor you and add toxins - then is it really ‘treatment’ or is it “Treatment TM”?

The underlying cause of cancer that needs to be corrected for health to return normally, is quantum proton tunneling of healthy cytoplasm - structured water of life with a good balance of electrolytes for us to maintain our mini ocean of life. We are marine creatures who evolved to carry our ocean around with us. The miracle of pregnancy is that the uterus is an inland sea for the fetus as it grows through its tadpole and other phases of development. A plastic surgeon or prenatal incubator biotech company is NEVER going to come anywhere close to what a woman’s body can do. Good nourishment and reduced stress helps, but the miracle of life chugs on in difficult settings too.

• See: Nature loves a good design; Cancer, POTS, Epigenetics & the One-Carbon Methylation Cycles. (substack.com)

• And: Cancer care or profit stream? Story of Dr. Burzynski and an ancient therapy (urine!). (substack.com)

Iodine, magnesium, potassium, calcium, sodium, phosphorus and other trace minerals all help our electrical body. Some call it chi, others call it aether/orgone or other things - the energy of life performs at quantum speed during health and doesn’t during chronic illness.

Gentle stretching and rhythmic motions help us gather more aether energy from within and from our surroundings. The aura bubble of energy surrounding us, spoken of in some alternative circles, is accurate - and has been photographed with Kirlian techniques. This explainer says it is water reacting with the electrical photographic imaging technique - no standard camera is used, just the special photo imaging paper reacting with energy directly. This sounds like aether energy is being measured - it would be around live objects or non-live things and is attracted to water and magnetic attracting metals. See: What is Kirlian Photography, the Science and Myth Revealed. (lightstalking.com)

Why seek “Treatment TM” from people who don’t understand health? Prevention is needed though, waiting until the body is very ill is more of an up-hill battle.

Part 1: microRNA, mRNA, CoV and cancer, April 28, 2023, (substack.com).

Part 2 - microRNA, mRNA, CoV injections & cancer; Baicalin resource by David Cowley and Johanna Deinert, April 29, 2023, (substack.com). *This has a Reference List of the links included (most) by Annoyed Citizen in his post, (substack.com).

Part 3 - microRNA, mRNA, CoV and Cancer; miR-21 and PPARa, RXR, palmitate or glucose versus polyphenols, May 1, 2023, (substack.com). **This post has an intro that summarizes the series and provides links to articles being discussed.

Part 4. modRNA, pseudouridine, snoRNA; Cancer, CoV, microRNA, mRNA, May 2, 2023, (substack.com)

Part 5. Annoyed Citizen Interview with Kevin McCairn & a preprint re jab effects on innate and adaptive immune system (substack.com)

Some quotes and links from Annoyed Citizen’s post - Der Weg Richtung Himmel oder Hölle - by Genervter Bürger, (substack.com).

Near email length. Guten tag!

Things to avoid:

Hazardous Chemicals that are FDA Approved, by Food Babe, Vani Hari. I do like her inclusion of High Fructose Corn Syrup.

Disclaimer: This information is being shared for educational purposes within the guidelines of Fair Use and is not intended to provide individual health guidance.

Reference List

(Balcerak, et al., 2019) Balcerak, A., Trebinska-Stryjewska, A., Konopinski, R., Wakula, M., Grzybowska, E.A., (2019). RNA–protein interactions: disorder, moonlighting and junk contribute to eukaryotic complexity, Open Biol. 9 190096190096 http://doi.org/10.1098/rsob.190096, https://royalsocietypublishing.org/doi/10.1098/rsob.190096

(Granados-Riveron, Aquino-Jarquin, 2021) Granados-Riveron JT, Aquino-Jarquin G. Engineering of the current nucleoside-modified mRNA-LNP vaccines against SARS-CoV-2. Biomed Pharmacother. 2021 Oct;142:111953. doi: 10.1016/j.biopha.2021.111953. Epub 2021 Jul 23. PMID: 34343897; PMCID: PMC8299225. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299225/ via https://twitter.com/NarfGb/status/1656177162037211137?s=20

(Milenkovic, Jude, Morand, 2013) Milenkovic, D., Jude, B., Morand, C., (2013). ‘miRNA as molecular target of polyphenols underlying their biological effects’. Free Radic Biol Med. Sep;64:40-51. doi: 10.1016/j.freeradbiomed.2013.05.046. Epub 2013 Jun 7. PMID: 23751562. https://www.sciencedirect.com/science/article/abs/pii/S0891584913002748?via%3Dihub