Part 5. Annoyed Citizen Interview with Kevin McCairn & a preprint re jab effects on innate and adaptive immune system
The series is based on links shared by Annoyed Citizen, Generveter Burger in his native language German, an anonymous name for social media and on Substack. He and another anon and Substacker, Doorless Carp, spoke on Kevin McCairn’s livestream a few days ago, about some of the articles in his post and others.
It is nice to hear Annoyed Citizen’s voice and concern. He has stressed that he is a lay reader and is learning. He shares articles that he finds concerning and it is up to us to read more and look into it more. The information is frightening and seems important so let's look at it closer — all are good points. So, let's continue to take a closer look.
The Molecular Bomb: Why Nuking miRNA Signaling With Synthetic mRNA Is A bad Idea, livestream by kevin_w_mccairn_phd Kevin McCairn, PhD, with guests Annoyed Citizen and Doorless Carp (twitch.tv): (twitch.tv)
Innate and adaptive immune systems - in brief. The innate immune system is our frontline defense - our skin and membranes and glycocalyx layer coating the intestinal lining. It includes general defense white blood cells called Scavenger Cells that engulf pathogen or cellular debris and Natural Killer cells that recognize larger groups of pathogenic proteins and remove infected cells. The adaptive immune system includes the B memory cells that make more specific antibodies against pathogen proteins and T cells. See: The innate and adaptive immune systems - InformedHealth.org - NCBI Bookshelf (nih.gov)
My series is about a post by Annoyed Citizen: Der Weg Richtung Himmel oder Hölle - by Genervter Bürger (The Road to Heaven or Hell, by Annoyed Citizen) (substack.com)
Part 1: microRNA, mRNA, CoV and cancer, April 28, 2023, (substack.com).
Part 2 - microRNA, mRNA, CoV injections & cancer; Baicalin resource by David Cowley and Johanna Deinert, April 29, 2023, (substack.com). *This has a Reference List of the links included (most) by Annoyed Citizen in his post, (substack.com).
Part 3 - microRNA, mRNA, CoV and Cancer; miR-21 and PPARa, RXR, palmitate or glucose versus polyphenols, May 1, 2023, (substack.com). **This post has an intro that summarizes the series and provides links to articles being discussed.
Part 4. modRNA, pseudouridine, snoRNA; Cancer, CoV, microRNA, mRNA, May 2, 2023, (substack.com)
Starting with an article from early in the interview:
A preprint article is discussed in the livestream which I missed or was not in the post: ‘The impact of BNT162b2 mRNA vaccine on adaptive and innate immune responses,’ (Föhse, et al., 2023).
A small cohort of mostly health care professionals had immune biomarkers measured before and after BNT162b2 mRNA vaccinations 1 and 2 and a 3rd booster at a year. The goal was to assess the effects of the mRNA type ‘vaccine’ on the innate and adaptive immune responses against SARS-CoV-2 variants and other pathogens. Whether Varicella zoster (Shingles/Chickenpox) is being reactivated is also discussed. T-cell effects were noted, and increases in some inflammatory cytokines (IL-1/IL-6, IL-1Ra, following a pathogen exposure but interferon response was reduced.
We must keep in mind that authors are probably being cautious in their wording in the hopes of getting published. It is a Preprint currently. The tone of this article is mixed – are they for these injections or not? It is not really obvious – yet they wrote this:
“Our results may explain the data from a recent study of over 50,000 healthcare workers that found that the more doses of the mRNA vaccine received by the individuals, the higher their risk of contracting COVID-19 . It may be thus hypothesized that vaccination with mRNA-based vaccines causes dysregulation of innate immune responses, and that the consequences of this effect for protection against SARS-CoV-2 cannot be fully compensated by the induction of adaptive immune responses.” (Föhse, et al., 2023)
More excerpts, paragraph breaks added by me:
“Similar changes in the production of cytokines and interferons accompany the transcriptional responses to viral stimulation after vaccination. In this respect, the production of the cytokines from the IL-1/IL-6 pathway, including the anti-inflammatory IL-1Ra, tended to increase six months after the first vaccination.
More remarkable is, however, the tendency of lower interferon responses after BNT162b2 vaccination. BNT162b2 vaccination has been previously reported to activate virus-specific CD4+ and CD8+ T cells and upregulate the production of immune-modulatory cytokines such as IFN-γ shortly after primary and secondary vaccination [5,25–27].
In contrast to these studies, we observed a downregulation of the type I interferon pathway in response to influenza at the transcriptional level, and lower IFN-α production by PBMCs after stimulation with SARS-CoV-2. A similar pattern can be observed for IFN-γ, which was produced less by PBMCs after immunization when exposed to various viral stimuli. The cause of these different findings is unclear, although using different methodologies may partially explain them. Samanovic et. al., for instance, assessed the percentage of IFN-γ+ CD4+, and CD8+ T cells in response to the spike-peptide mix after a relatively short stimulation , whereas we measured the secreted cytokine in response to the heat-inactivated virus after a 7-days stimulation period.” (Föhse, et al., 2023)
And the excerpt that includes the shorter quote:
“Our results may explain the data from a recent study of over 50,000 healthcare workers that found that the more doses of the mRNA vaccine received by the individuals, the higher their risk of contracting COVID-19 . It may be thus hypothesized that vaccination with mRNA-based vaccines causes dysregulation of innate immune responses, and that the consequences of this effect for protection against SARS-CoV-2 cannot be fully compensated by the induction of adaptive immune responses.
On the other hand, the more dampened transcriptional reactivity of the immune cells to secondary viral stimulation (immune tolerance) may provide an explanation for the protective effects of BNT162b2 against severe COVID-19. Overwhelming inflammation is one of the important pathological features in patients with COVID-19. Thus, a more regulated inflammatory response may explain why vaccination had especially effects on the reduction of disease severity in case of the delta and omicron variants, rather than a full protection against infection . Indeed, the complete absence of neutralization capacity against omicron in this and other studies argues that cellular mechanisms, such as other T-cell-mediated or innate immune cell-mediated pathways, are responsible for these effects.” (Föhse, et al., 2023)
Reminder again that zinc deficiency alone can lead to elevated IL-6 in older people. More zinc is needed than guidelines suggest in order to maintain thymus gland function which is where immune T cells and B cells are produced. See: Zinc Deficiency and elevated IL-6 > mast cell degranulation>histamine excess; also >microbiome dysbiosis. “The lack of zinc leading to lack of DNA methylation leads to age related effect of increased production of the inflammatory cytokine IL-6 (which can be elevated in COVID19). IL-6 degranulates mast cells, which adds to histamine excess risk and releases more inflammatory cytokines.” - Jennifer Depew, RD, (substack.com)
MicroRNA up or down regulation may be a biomarker of disease. MiRNA-21 up-regulation is seen in lymphoma.
Returning to microRNA and links in Annoyed Citizen’s post - MicroRNA that are up or down regulated may be useful as biomarkers of disease. While many microRNA changes have been identified in patients with different types of cancer or the same type – which are modified has varied between research teams and sometimes one that is upregulated in one patient or study was down-regulated in others. MicroRNA-21 was more consistently seen to be up-regulated in a type of lymphoma – within six out of eight studies. (Lopez-Santillan, et al., 2018)
The following graphic summarizes microRNAs seen in lymphomas with altered expression.
That graphic seems overwhelming and probably frightening, it was shared in the post by Annoyed Citizen via JikkyLeaks mentioned as the source. It is compelling — sooooo many microRNA can promote cancer when present in excess or in reduced levels - either up-regulated or down-regulated.
“Never fear, the plant world is near!” - cartoon Super Underdog, paraphrased.
The plant world and its phytonutrients can reset the balance of microRNA by up or down regulating it back to normal. And can affect many, many at the same time, see Figure 2 image below. “Figure 2.ROS-mediated anti-cancer activities associated with miRs regulated by polyphenols. miRs in red and in blue are upregulated and downregulated by polyphenols, respectively.” (Yoshioka, et al., 2022)
Plants are so awesome, because they want to survive and they need to respond quickly to a sudden cold spell, or drought, or blazing sunshine with no cloud break in sight. That requires being responsive to change chemically.
Too cold and cloudy? The spring anemones won't open their petals. Sunflowers turn towards the sun. Like magic? Or like microRNA changes? I am not sure of the specifics on following the sun but TRP channels are likely involved - sensing more warmth and light in one side of the plant compared to the other side. How the turning occurs? I don't know.
Underdog had a Super Energy pill when he needed to recharge his powers. Methyl B complex and polyphenols I hope, not just caffeine, the coffee polyphenols count too. Good rich dark coffee has more of the bitter phytonutrients that can help us.
Polyphenols in our food and beverages
Star players include genestein in soy, EGCG in green tea or pomegranate peel, curcumin in turmeric used in curry seasoning, quercetin in onion, resveratrol in red wine and chlorogenic acid in coffee. See Table 1 by Yoshioka, et al., 2022.
The microRNA that may be up or down regulated by the phytonutrients are shown in Figure 2 by Yoshioka, et al., 2022.
Never fear! Supercharge your diet with plants instead!
Nearing email length limit.
Why care about microRNA in relation to CoV?
SARS-CoV-2 is an RNA based virus and also has messenger and microRNA.
Spike mRNA: "The S mRNA collapses with the S protein and supports the translocation of the S mRNA into the nucleus. While the translocation of the nucleoprotein (N) into the nucleus has been demonstrated in many coronaviruses, the translocation of both the S-mRNA and the S-protein in the nucleus is a new feature of SARS-CoV-2." (Sattar, et al., 2023)
Spike miRNA: "The calculation showed that sixteen SARS-CoV-2-S-derived miRNAs bound to the negative S-RNA strand with fairly strong avidity. In addition, CovS-miR-21 downregulated Rho-associated coiled-coil-containing protein kinase (ROCK2) and aryl hydrocarbon receptor nuclear translocator (ARNTL), and CovSmiR-3 decreased lysine methyltransferase 2C (KMT2C). Therefore, in the METS analysis, CovS-miR-21 suppressed Ras homologue family member A (RhoA)/Rock2 signaling function and circadian rhythm, and CovS-miR-3 inhibited histone H3-K4 methylation.
We found that BNT162b2 inhibits the replication of SARS-CoV-2 by degrading viral negative-stranded RNAs fully paired with SARS-CoV-2 S-derived miRNAs. In addition, CovS-miR-162 derived from BNT2b21 restores circadian rhythm and attenuates immunogenicity. Quantum miRNA assessments showed that the BNT162b2 RNA vaccine has the character of a miRNA vaccine and is an excellent vaccine with high efficacy and low side effects." (Fujii, 2021)
Quotes from Annoyed Citizen’s post - Der Weg Richtung Himmel oder Hölle - by Genervter Bürger, (substack.com), more on the topic later. Salutations!
Disclaimer: This information is being shared for educational purposes within the guidelines of Fair Use and is not intended to provide individual health guidance.
(Drillis, et al., 2021) Drillis, G., Goulielmaki, M., Spandidos, D.A., Aggelaki, S., & Zoumpourlis, V. (2021). Non‑coding RNAs (miRNAs and lncRNAs) and their roles in lymphogenesis in all types of lymphomas and lymphoid malignancies (Review). Oncology Letters, 21, 393. https://doi.org/10.3892/ol.2021.12654 https://www.spandidos-publications.com/10.3892/ol.2021.12654
(Föhse, et al., 2023) Föhse,K., Geckin, B., Zoodsma, M., Kilic, G., Liu, Z., Röring, R.J., et al., (2023), ‘The impact of BNT162b2 mRNA vaccine on adaptive and innate immune responses,’_medRxiv 2021.05.03.21256520; doi: https://doi.org/10.1101/2021.05.03.21256520, This article is a preprint and has not been peer-reviewed [what does this mean?]. It reports new medical research that has yet to be evaluated and so should not be used to guide clinical practice. https://www.medrxiv.org/content/10.1101/2021.05.03.21256520v2 It is made available under a CC-BY-NC-ND 4.0 International license; this version posted March 27, 2023.
(Lopez-Santillan, et al., 2018) Lopez-Santillan M, Larrabeiti-Etxebarria A, Arzuaga-Mendez J, Lopez-Lopez E, Garcia-Orad A. Circulating miRNAs as biomarkers in diffuse large B-cell lymphoma: a systematic review. Oncotarget. 2018 Apr 27;9(32):22850-22861. doi: 10.18632/oncotarget.25230. PMID: 29854319; PMCID: PMC5978269. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978269/
(Yoshioka, et al., 2022) Yoshioka Y, Ohishi T, Nakamura Y, Fukutomi R, Miyoshi N. Anti-Cancer Effects of Dietary Polyphenols via ROS-Mediated Pathway with Their Modulation of MicroRNAs. Molecules. 2022 Jun 14;27(12):3816. doi: 10.3390/molecules27123816. PMID: 35744941; PMCID: PMC9227902. https://www.dropbox.com/s/npdritgmgwf6987/molecules-27-03816-v3.pdf?dl=0
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