microRNA, mRNA, CoV and cancer

Pomegranate would help.

microRNA have regulatory control over whether mRNA will be broken down or used to make a protein. Levels of microRNA that are up or down regulated may have ripple effects that can lead to cell proliferation and cancer promotion. The CoV injections are supposed to make chimeric spike protein from mRNA that encodes for it - which the BioNTech and Pfizer companies said it would. But can they prove it does? Have they proved it? What they have released suggests they don’t consistently make the correct protein, and that the percent correct got worse from the clinical trial version to the mass-produced version - from 78% correct length segments down to 55% correct.

What are the wrong length fragments doing? We don’t really know what they are or whether they would be transfected and lead to a protein being produced.

What the ‘blots’ on a gel electrophoresis plate image provided by BioNTech/Pfizer suggest is that they may have committed fraud about the purity of their product and provided faked gel plate images. Fraudulent work would negate the liability free clause of their contracts. See: Startling Evidence Suggests BioNTech and Pfizer Falsified Key Data: Part 1, by sonia_elijah, Feb 4, 2023, (trialsitenews.com), Excerpts are included later - see the article for images from the Pfizer documents though, and a quote of Jikkyleaks.

Part 2 gets into the wrong length question. Part 2: Startling Evidence Suggests BioNTech/Pfizer Falsified Key Data & Further Scandals (trialsitenews.com)

My source for the article and more info I haven’t mentioned yet is a Substacker: Title translated into English, from the original German: The Road to Heaven or Hell - by Genervter Bürger = “Annoyed Citizen” (substack.com)

Der Weg Richtung Himmel oder Hölle

Two videos describing microRNA and an article are in English, and it can be auto-translated.

The article is Part 1 of a doozy - #BlotGate → How do we know that the mRNA in the BioNTech and Pfizer LNPs is correct for making the proposed chimeric spike protein? They admitted to it only being 55% correct in the production batches, a big drop from 78% in the clinical trial batches - what if it was even worse? what if the other 45% or more wrong mRNA is also able to lead to protein production in a human recipient’s cell?

“Last June, Trial Site News broke the scandal of the leaked European Medicines Agency emails and confidential Pfizer/BioNTech related documents, with an in-depth analysis and a follow up report.

Damning details in both reports exposed how key regulators such as the EMA, FDA, Health Canada and the MHRA were fully aware of the significant drop in RNA integrity (which is a critical quality attribute) to ~55% in the commercial batches (Process 2: large scale production) of the Pfizer-BioNTech Covid-19 vaccine compared to ~78% in the clinical batches (Process 1: small scale production).”

[…]

“A document from a pivotal meeting of November 26, 2020, between the regulator (EMA) and Pfizer/BioNTech revealed the alarming fact that this “major objection” was “solved” by simply lowering the standard down to 50% even when Pfizer claimed, “The efficacy of the drug product is dependent on the expression of the delivered RNA, which requires a sufficiently intact RNA molecule.” Furthermore, the level set was significantly lower than the minimum threshold of 70% that Acuitas Therapeutics had stipulated.”

Startling Evidence Suggests BioNTech and Pfizer Falsified Key Data: Part 1, by sonia_elijah, Feb 4, 2023, (trialsitenews.com)

If the companies were found to have made fraudulent claims about their product quality then the liability protection would be void. — Is the premise I believe. Startling Evidence Suggests BioNTech and Pfizer Falsified Key Data: Part 1, by sonia_elijah, Feb 4, 2023, (trialsitenews.com) *Site requires registration for up to 10 free articles a month without a subscription.

Excerpts: “Several Western Blot tests were conducted to evaluate the protein expression of the mRNA in HEK cells transfected with the vaccine taken from different lots. Using this technique, the expressed proteins showed up as highly unusual looking ‘bands.’

Certain independent scientific experts have described these Western blots as the “smoking gun” evidence (particularly the “duplication” of the results) which suggest that BioNTech and Pfizer falsified key data as part of their submissions to the European Medicines Agency and the US Food and Drug Administration  for securing emergency use authorisation (conditional) and later marketing authorisation approval of their product.

[…]

The fraudulent-looking data provided by BioNTech/Pfizer with regards to the quality of their novel mRNA vaccine also raises other salient questions:

1. How did the “copy and paste” data sail through the radar of the regulators?

2. How did the Journal of Pharmaceutical Sciences publish the same fraudulent looking data presented in a Pfizer-funded paper, written by Pfizer and BioNTech’s employees (Patel et al.)?

3. What proteins are being expressed in human cells from the vaccinal modified mRNA, other than the SARS-CoV-2 spike protein?

4. Why has no genomic sequence of the expressed protein of the mRNA vaccine ever been published?

Startling Evidence Suggests BioNTech and Pfizer Falsified Key Data: Part 1, by sonia_elijah, Feb 4, 2023, (trialsitenews.com)

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MicroRNA control most of everything else. If the spike or other fragments in the CoV jabs are affecting microRNA than a large ripple effect can occur, more of a tsunami of escalating damage. So many things can be affected by the same microRNA types. Spoiler for good news - plant polyphenols can change lots of microRNA in beneficial ways - resetting negative levels to health promoting levels.

microRNA background info, article has a paywall:

From the Abstract:

“miRNAs can act as epigenetic modulators by targeting key enzymes responsible for epigenetic reactions such as DNA methyltransferases (DNMTs), histone deacetylases (HDACs) and histone methyltransferases (EZH) [7, 8, 9]. Moreover, the expression of miRNAs is also regulated by epigenetic machinery, including DNA methylation, RNA modification and histone modification.

The reciprocity relationship between miRNAs and epigenetic regulation forms the miRNA-epigenetic feedback loop (Figure 1).

The modulation of miRNA-epigenetic feedback loop and its cellular function has emerged as a novel mechanism of regulating cell process, including cell proliferation [10^•], apoptosis [11], and differentiation [12].” (Yao, Chen, Zhou, 2019) *There is a paywall.

via @carl_jurassic · 5h “Excellent review of miRNA roles in epigenetic regulation”

When there is a feedback loop - regulatory feedback - then dysregulation can escalate into super dysregulated (not a sciencey term) function, out of control function.

“The reciprocity relationship between miRNAs and epigenetic regulation forms the miRNA-epigenetic feedback loop (Figure 1).” (Yao, Chen, Zhou, 2019)

The Indiana Jones gif to apply would be the giant stone ball rolling towards him as he runs away from the ancient trap that he set off. Normal control is gone, miRNAs may lead to escalated protein production and out of control growth or some other ill effect.

“The reciprocal actions of miRNAs and epigenetic pathway appear to form a miRNA-epigenetic feedback loop and have an extensive influence on gene expression proliferation. The dysregulation of the miRNA-epigenetic feedback loop interferes with the physiological and pathological processes and contributes to variety of diseases.” (Yao, Chen, Zhou, 2019) *Same article as earlier excerpts.

The use of “miRNA-epigenetic feedback loop” as a science phrase seems new, not many search results besides the initial paper.

An example is in a different article about a type of lymphoma cancer and a microRNA, miR-518a-5p, that seems involved in its regulation as a negative feedback in tandem with CCR6 (C-C chemokine receptor (CCR)6). Down-regulating the cancer growth.

“We found that negative correlation existed between CCR6 and miR-518a-5p in DLBCL. Both up-regulated miR-518a-5p and down-regulated CCR6 inhibited cell proliferation and invasion in vitro. Experiment then verified the regulatory relationship between miR-518a-5p and CCR6. […]

Thus, these findings indicated that miR-518a-5p and CCR6 formed a negative regulatory feedback loop in [diffuse large B cell lymphoma] DLBCL.” (Huang, Zhang, Fu, Shen, 2021) *No paywall.

Why science is confusing - a tangent:

This protein is known by four names; I rest my case, that is all:

• “(1.) Chemokine (C-C motif) ligand 20 (CCL20), also known as

• (2.) macrophage inflammatory protein (MIP)-3α,

• (3.) liver activation regulated chemokine (LARC), and

• (4.) Exodus-1,

• is a small protein that is physiologically expressed in the liver, colon, and skin, is involved in tissue inflammation and homeostasis,

• and has a specific receptor C-C chemokine receptor 6 (CCR6).”

• (Kadomoto, Izumi, Mizokami, 2020) *No paywall, (#.)s added by me.

Tangent going further “Chemokines, which are basic proteins that exert their effects via G protein-coupled receptors and a subset of the cytokine family, are mediators deeply involved in leukocyte migration during an inflammatory reaction.” (Kadomoto, Izumi, Mizokami, 2020)

Unrelated to CCR6 which only has one known ligand, CCL20. Did you know that bitter taste receptors are a type of G-protein coupled receptor? So is niacin/butyrate GP109 and endocannabinoid receptors too. G-protein coupled receptors are like cell membrane machines that can receive a message outside, like a doorbell buzzer, and have actions occur inside, like set off protein production in the cell nucleus by starting a series of chemical steps.

Tying a bow out of the loose ends - there seems to be co-regulation between microRNA, chemokines, and G-coupled receptors, which likely include bitter taste receptors too based on the astounding efficacy of polyphenols found in pomegranate or green tea against cancer. The details of precisely how bitter taste receptors may have co-regulatory roles affecting weight, cancer, and other health issues needs more research.

“Bitters” has been a medicinal tonic for a long time and medical research needs to catch up. Traditional Chinese Medicine uses many bitter tasting herbal blends, but how bitter taste receptors are helping us as functional cell machines found in organs besides the tongue needs more research.

The tongue is our personal dosing device - pay attention and the body can learn what tastes help which symptoms and how much to eat before stopping because it now doesn’t taste as wonderful as the initial impression. Our tongue will like what we need and get overstimulated as we eat enough of it and then the food will seem bland or even bad - the same food.

looking for ‘microRNA & bitter taste receptors’ found an article about SARS/covid that is missing something important about bitter taste receptors:

“Question  What is the association between the bitter taste receptor phenotype and outcomes after infection with SARS-CoV-2?

Findings  In this cohort study of 1935 adults, 266 tested positive for SARS-CoV-2, and those who experienced low intensity of bitter tastes or no bitter tastes (nontasters) were significantly more likely to test positive for SARS-CoV-2, to be hospitalized, and to be symptomatic for a longer duration. Conversely, those who experienced greater intensity of bitter tastes (supertasters) represented 5.6% of patients infected with SARS-CoV-2, suggesting enhanced innate immune protection.

Meaning  This study suggests that bitter taste receptor allelic variants are associated with innate immune fitness toward SARS-CoV-2 and can be used to correlate with clinical course and prognosis of COVID-19.” (Barham, et al., 2021)

This “Meaning”/Conclusion statement “This study suggests that bitter taste receptor allelic variants are associated with innate immune fitness toward SARS-CoV-2…” is making too big of a guess - people can have low bitter taste sensitivity due to being zinc deficient and may have nothing to do with individual gene allele variants. They should have just reported what they had observed - people with low bitter taste sensitivity were more at risk for SARS infection. So, let’s find out why those people have low bitter taste sensitivity - and treat them for it!

Being zinc deficient and without bitter taste receptors would certainly increase your risk of infection with more severe symptoms as bitter taste receptors help prevent asthmatic congested lungs. That is why citrus peel helps so much - anti-asthmatic and anti-viral. Zinc is needed to make bitter taste receptors and other proteins within Zinc Finger Proteins which help transcribe many different genes into proteins - including taste receptors of any type.

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I wandered from #BlotGate and microRNA - the “BlotGate” is referring to the way labs look for and differentiate between genes or other proteins. A seaweed gelatin mix is made and chilled in a rectangular tray. Samples of unknown proteins can be injected into one side and the proteins move up through the gelatin on the tray towards the other side - how far they make it across shows different length stripes or blots on the gel as the sample has a pigmented dye added or some other method to make it visible like gene transfection with glowing luciferase.

https://blog.universalmedicalinc.com/using-agarose-in-gel-electrophoresis/ image source and brief info about Using Agarose in gel electrophoresis.

The CoV injections had too many different stripes/blots - it isn’t a pure product and lying to say it was pure enough should invalidate any contracts made with BioNTech or Pfizer.

The health concern is the HUGE unknown - What mRNA is present? What proteins might incomplete fragments make? Or are there smaller fragments that may even be acting like microRNA and having bigger effects on other mRNA and its protein production? We don’t know. There is no official inquiry.

**I am not done reading all the articles in Genervtor’s Substack, so maybe this is my Part 1.

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I listened to a Townhall phone call by the AARP yesterday - it was 30 minutes or more and callers could ask questions - but it all seemed very staged. Supposedly about the end of the CoV pandemic, yeah! NO, fear it still, get your “safe and effective” bivalent boosters. Masking is probably still a good idea, testing for CoV if exposed, still a good idea, and Avian Flu may be the next pandemic, let’s be excited/fearful about that, shall we? A recording is supposed to be on their website aarp.org/covidtownhall < not working yet

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Whatever the question, pomegranate is often a good answer. Modulators fix things whether up or down regulation is needed. They are the dimmer switch for sensitive eyes - producing Goldilock’s (Baby Bear’s) perfectly warm, not too hot or too cold, porridge.

• Effect of Pomegranate Extract in Mesenchymal Stem Cells by Modulation of microRNA-155, microRNA-21, microRNA-23b, microRNA-126a, and PI3K\AKT1\NF-[Formula: see text]B Expression. (Rostami, et al., 2020)

Disclaimer: This information is being shared for educational purposes within the guidelines of Fair Use and is not intended to provide individual health guidance.

Reference List

(Barham, et al., 2021) Barham HP, Taha MA, Broyles ST, Stevenson MM, Zito BA, Hall CA. Association Between Bitter Taste Receptor Phenotype and Clinical Outcomes Among Patients With COVID-19. JAMA Netw Open. 2021;4(5):e2111410. doi:10.1001/jamanetworkopen.2021.11410 https://jamanetwork.com/journals/jamanetworkopen/fullarticle/2780134

(Huang, Zhang, Fu, Shen, 2021) Huang Q, Zhang F, Fu H, Shen J. Epigenetic regulation of miR-518a-5p-CCR6 feedback loop promotes both proliferation and invasion in diffuse large B cell lymphoma. Epigenetics. 2021 Jan;16(1):28-44. doi: 10.1080/15592294.2020.1786317. Epub 2020 Jun 30. PMID: 32600091; PMCID: PMC7889247. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889247/

(Kadomoto, Izumi, Mizokami, 2020) Kadomoto S, Izumi K, Mizokami A. The CCL20-CCR6 Axis in Cancer Progression. Int J Mol Sci. 2020 Jul 22;21(15):5186. doi: 10.3390/ijms21155186. PMID: 32707869; PMCID: PMC7432448. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432448/

(Rostami, et al., 2020) Rostami Z, Khorashadizadeh M, Ghoncheh M, Naseri M. Effect of Pomegranate Extract in Mesenchymal Stem Cells by Modulation of microRNA-155, microRNA-21, microRNA-23b, microRNA-126a, and PI3K\AKT1\NF-[Formula: see text]B Expression. DNA Cell Biol. 2020 Oct;39(10):1779-1788. doi: 10.1089/dna.2020.5775. Epub 2020 Aug 31. PMID: 32865424. https://pubmed.ncbi.nlm.nih.gov/32865424/

(Yao, Chen, Zhou, 2019) Qian Yao, Yuqi Chen, Xiang Zhou, 2019, The roles of microRNAs in epigenetic regulation, Current Opinion in Chemical Biology, 51:11-17, ISSN 1367-5931, https://doi.org/10.1016/j.cbpa.2019.01.024. https://www.sciencedirect.com/science/article/abs/pii/S1367593118301868