Part 2 - microRNA, mRNA, CoV injections & cancer; Baicalin resource by David Cowley and Johanna Deinert

Post too long for email

Addition - this post focuses on the Part 2 article linked in my last post, Excerpt:

What the ‘blots’ on a gel electrophoresis plate image provided by BioNTech/Pfizer suggest is that they may have committed fraud about the purity of their product and provided faked gel plate images. Fraudulent work would negate the liability free clause of their contracts.

• See: Startling Evidence Suggests BioNTech and Pfizer Falsified Key Data: Part 1, by sonia_elijah, Feb 4, 2023, (trialsitenews.com), Excerpts are included later - see the article for images from the Pfizer documents though, and a quote of Jikkyleaks.

• Part 2 gets into the wrong length question. Part 2: Startling Evidence Suggests BioNTech/Pfizer Falsified Key Data & Further Scandals (trialsitenews.com) Includes more images from the Pfizer documents and refers to work by Jikkyleaks and by Kevin McKernan.

~~

The BioNTech/Pfizer data not only included what seemed like a computer copy/paste job on automated types of gel electrophoresis images (Part 1 article above) but also included two of the more traditional images of an actual gel plate with fuzzier ‘bands’ showing how far a group of similar proteins traveled up the gel plate. (Part 2 article above.)

The actual gel plate images do have extra bands suggesting fragmented proteins were present. The company’s report didn’t mention the extra bands or provided explanations that had gaps. They showed that the fragments wouldn’t make S1/S2 spike protein by testing it with antigens for those two proteins, but they didn’t do any sequencing to identify the unknown fragments or to see if any unknown proteins had been produced - had a fragment led to transfection of a cell leading to production of the mRNA fragment?

Another research team, (Patterson, et al., 2022, preprint), did show that CoV injected people were expressing proteins that were not the same as the chimeric spike sequence. The team studied fifty patients who had received CoV injections but never had a reported CoV infection and who did develop symptoms similar to LongCovid, also now known as PASC, post-acute sequelae SARS-CoV-2 infection (PASC), after the injection. The research team tested the participants for antibodies against the spike nucleocapsid protein to show that they hadn’t had the viral infection or used an FDA T-Detect Test.

“Lack of prior infection was determined using anti-nucelocapsid antibody testing or Food and Drug Administration (FDA) Emergency Use Authorization (EUA) T-Detect Test” (Patterson, et al., 2022, preprint)

Thirty-five people who were healthy 90 days after CoV injection were used as a Control Group. *Potential flaw in the research design - we don’t know that all the injected people got mRNA LNPs or whether some got saline injections. I don’t see anywhere in the paper where they say they tested the 35 controls for presence of nucleocapsid and S1 or S2 protein antigens - to prove whether the control group had had a previous infection or whether they did respond to the CoV injections with spike production.

Ten of the control group were included in more extensive testing which found that the people with LongCovid/PASC symptoms were producing S1, S2 and other unknown but similar ‘mutant’ proteins. Figure 1 shows that the participants with LongCovid symptoms were SICK, not faking it. Compared to the control group who had a little IL-6 elevation**, the LongCovid group had consistently elevated IL-6, IL-8, CCL5, and sCD40L, with some lower numbers. Figure 2 shows that a few of the Control group did exhibit a small amount of S1 but many had zero. Figure 3B shows “Potential mutant S1 peptides” - found in the LongCovid group. (Patterson, et al., 2022, preprint)

• **Tangential News to Know - anyone who is low in zinc and of older age, is likely to have elevated IL-6. (Previous post, see below.)

“Analysis of S1 Protein Persistence

Similar to our published findings of S1 persistence in non-classical and intermediate monocytes in PASC 9 , we investigated whether a similar mechanism could account for the PASC-like symptoms in postvaccination individuals with PASC-like symptoms. We used flow cytometry to screen patients for S1 protein in their monocyte subsets. We screened 14 post-vaccination individuals with simultaneously collected PBMCs and with PACS-like symptoms and 10 normal controls. As demonstrated in figure 4, there was statistically significant elevation of S1 containing non-classical monocytes (13 of 14, P<0.001) and S1 containing intermediate monocytes (9/14, P=0.006).

Of the S1 positive post-vaccination patients, we sorted the CD16+ cells from six patients as previously performed for PASC patients 9 . Upon isolation of the protein, we demonstrated by LC-MS that these CD16+ cells from post-vaccination patients also contained S1 protein months after vaccination (Fig 5A).

Further analysis revealed that these S1 positive, CD16+ cells also contained peptide sequences of S2, and mutant S1 peptides (Fig 5B).” (Patterson, et al., 2022, preprint)

(Patterson, et al., 2022, preprint) SARS-CoV-2 S1 Protein Persistence in SARS-CoV-2 Negative Post-Vaccination Individuals with Long COVID/ PASC-Like Symptoms 206f2ad5-a550-4359-a37e-18170828137a.pdf (researchsquare.com)

Spike S1 and mutated spike proteins were found in LongCovid/PASC patients. Without gene sequencing of the unknowns we won't know what they are or be able to look closer at what they may be doing. We can only see the patient’s symptoms.

Here we bumped into the too many names issue again - I had to look up what PASC stood for.

George Carlin and Soft Language come to mind - “PASC” sounds science-y and Very Important ~ We medical people know stuff about this condition that normal people can’t understand without a medical dictionary to look up the acronym - while “LongCovid” immediately tells you someone is feeling sick and that they think it is related to the Covid situation.

MIS-C is another Soft Language acronym, and ME/CFS.

• Related post - The S1 causes microvascular damage on its own: SPED fans - a Dec. 2020 Public Comment to the FDA. They were warned. (substack.com) The post has an excerpt from a letter written by a pediatric specialist who treats children with multisystem inflammatory syndrome (MIS-C). Fibrinolytics are highlighted as potential therapeutic aids an the prion like subsections of broken down spike is discussed with a comparison by me to the tessellation artwork of MC Escher. The spike misfolded protein tangles seem more interwoven, more compact and tough for the body to break down than previously seen protein tangles of amyloid or prion proteins.

• Related post 2 - Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), Houston, we have a problem. (substack.com)

*Reminder, prion proteins are a normal protein that we need to be doing its normal thing for us - when it is folded correctly. Hydration and trace mineral status can make a difference. The gel like cellular fluid helps stabilize the electrically active parts of folded proteins and may help keep them in their proper form. Plant phytonutrients also can help stabilize protein and mineral structures. Adequate magnesium is so important because it is a mineral that can stabilize a large cloud of water molecules around each atom of magnesium. Glyphosate is directly harmful to cellular matrix function as it affects the hook like protein at the end of tubular proteins like actin - if our internal scaffolding and crane can’t connect, then how can anything be built properly.

It helps make “science” less confusing to visualize that proteins and sugars and enzymes are all part of a tiny bustling factory, busy building things and interacting. Chemicals are like tiny machinery of a sort. A Tweet shared by Annoyed Citizen, The Road to Heaven or Hell - by Annoyed Citizen (substack.com), describes the role of P-53 in cancer suppression and calls proteins ‘nano-machines’ (ScienceWDrDoug) and a video shows in real time a simulation of a ribosome producing a protein from an mRNA with the three nucleotide sequences being matched to an incoming amino acid. It moves fast.

This post is too long for email,

…with the addition of a Reference List, see below, for Annoyed Citizen's post which goes much further into the question of what unknown RNA might be doing and what we know microRNa may do.

Additionally, Geoff Pain, PhD, shared in a reply that the Endotoxin added as an adjuvant alone may be causing liver failure due to microRNA 155 becoming upregulated by the Endotoxin and increasing in a positive feedback loop. Genetic susceptibility may be involved in that risk.

Rapid Liver Failure after Pfizer jabs most likely due to miR-155 upregulation by Endotoxin

Addressing the Reference List needs more space. A book length literature review about the health benefits of Baicalin, a phytonutrient extract of Scullaria/Skullcap, is included by Annoyed Citizen as a therapeutic possibility. (Cowley, Deinert, 2022) Like pomegranate peel, baicalin can inhibit the furin cleavage of the HIV gp120 protein which suggests it also would prevent the chimeric spike from separating into the S1 and S2 subunits.

The earlier zinc tangent led to a pat on my back - I found my own Substack at the top of the 3,800,000 other search results for ‘IL-6 zinc deficiency’. Nice SEO work, Substack.

• Zinc Deficiency and elevated IL-6 > mast cell degranulation>histamine excess; also >microbiome dysbiosis. “The lack of zinc leading to lack of DNA methylation leads to age related effect of increased production of the inflammatory cytokine IL-6 (which can be elevated in COVID19). IL-6 degranulates mast cells, which adds to histamine excess risk and releases more inflammatory cytokines.” - Jennifer Depew, RD, (substack.com)

Disclaimer: This information is being shared for educational purposes within the guidelines of Fair Use and is not intended to provide individual health guidance.

This is most but not all the links from Annoyed Citizen’s post, and I added one pomegranate link - I need to work with the list more now. Part 3…4, 5, and 6? <humor>

Reference List - from Annoyed Citizen’s post

(Alles, et al., 2019) Alles, J., Fehlmann, T., Fischer, U., Backes, C., Galata, V., Minet, M., et al., (2019). An estimate of the total number of true human miRNAs, Nucleic Acids Research, April 23:47(7):3353–3364, https://doi.org/10.1093/nar/gkz097

(Beibei, et al, 2020) Beibei, D., Feng, W., Xiang, N., Hengzhi, D., Yanru, Z, Zhongwei, Z., et al., (2020). The Cell Type–Specific Functions of miR-21 in Cardiovascular Diseases, Frontiers in Genetics, Vol. 11, DOI=10.3389/fgene.2020.563166 https://www.frontiersin.org/articles/10.3389/fgene.2020.563166/full

(Bortoletto, Parchem, 2023) Bortoletto AS, Parchem RJ. KRAS Hijacks the MicroRNA Regulatory Pathway in Cancer. Cancer Res. 2023 Mar 22:CAN-23-0296. doi: 10.1158/0008-5472.CAN-23-0296. Epub ahead of print. PMID: 36946612. https://pubmed.ncbi.nlm.nih.gov/36946612/

(Bracken, Scott, Goodall, 2016) Bracken, C., Scott, H., Goodall, G., (2016). A network-biology perspective of microRNA function and dysfunction in cancer. Nat Rev Genet 17, 719–732 (2016). https://doi.org/10.1038/nrg.2016.134 https://www.nature.com/articles/nrg.2016.134

(Brieño-Enríquez, et al., 2015) Brieño-Enríquez MA, García-López J, Cárdenas DB, Guibert S, Cleroux E, Děd L, et al. (2015) Exposure to Endocrine Disruptor Induces Transgenerational Epigenetic Deregulation of MicroRNAs in Primordial Germ Cells. PLoS ONE 10(4): e0124296. doi:10.1371/journal.pone.0124296 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0124296&type=printable

(Cannell, Kong, Bushell, 2008) Cannell, I.G., Kong, Y.W., Bushell, M., (2008). How do microRNAs regulate gene expression? Biochemical Society Transactions, Vol. 36(6) https://www.researchgate.net/profile/Ian-Cannell/publication/23485574_How_do_microRNAs_regulate_gene_expression/links/0912f50b3bec1aefdd000000/How-do-microRNAs-regulate-gene-expression.pdf

Baicalin - therapeutic for HIV furin cleavage.

(Cowley, Deinert, 2022) Cowley, D. and Deinert, J.P., 2022. Therapeutic Properties of Baicalin - A Literature Review [online]. 252 pp OSF Preprints, Available from: osf.io/2w8yn. “The Ukranians have known for at least 12 years that Baicalin (among other flavonoids) has furin inhibitory effects. Translated from Russian, a paper by Kibirev et al from back in 2010: (12)” Copy in myDropbox: https://www.dropbox.com/s/f2xmqqvnd1n6tja/Therapeutic%20properties%20of%20Baicalin_a%20literature%20review_DCowley.pdf?dl=0

(Duroix-Richard, et al., 2021) Duroux-Richard Isabelle, Apparailly Florence, Khoury Maroun, (2021). Mitochondrial MicroRNAs Contribute to Macrophage Immune Functions Including Differentiation, Polarization, and Activation, Frontiers in Physiology, Vol.12, DOI=10.3389/fphys.2021.738140 https://www.frontiersin.org/articles/10.3389/fphys.2021.738140/full

(Fujii, 2021) Fujii, Y.R., (2021). Quantum microRNA Assessment of COVID-19 RNA Vaccine: Hidden Potency of BNT162b2 SASR-CoV-2 Spike RNA as MicroRNA Vaccine, Crimsonpublishers.com https://crimsonpublishers.com/aics/fulltext/AICS.000552.php

(Hill, Tran, 2021) Hill M, Tran N. Global miRNA to miRNA Interactions: Impacts for miR-21. Trends Cell Biol. 2021 Jan;31(1):3-5. doi: 10.1016/j.tcb.2020.10.005. Epub 2020 Nov 11. PMID: 33189493. https://pubmed.ncbi.nlm.nih.gov/33189493/

(Huang, Guo, Li, 2020) Huang, B., Guo, X., Li, Y., (2020). lncRNA MALAT1 regulates the expression level of miR-21 and interferes with the biological behavior of colon cancer cells. J BUON. 2020 Mar-Apr;25(2):907-913. PMID: 32521885. https://www.jbuon.com/archive/25-2-907.pdf

(Jiang, Mei, 2021) Jiang, H., Mei, Y.-F., SARS–CoV–2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro. Viruses. 2021; 13(10):2056. https://doi.org/10.3390/v13102056 https://www.mdpi.com/1999-4915/13/10/2056

(Lin, et al., 2022) Lin Y-P, Hsieh Y-S, Cheng M-H, Shen C-F, Shen C-J, Cheng C-M. Using MicroRNA Arrays as a Tool to Evaluate COVID-19 Vaccine Efficacy. Vaccines. 2022; 10(10):1681. https://doi.org/10.3390/vaccines10101681 https://www.mdpi.com/2076-393X/10/10/1681

(Lu, et al., 2018) Lu, L., Xu, H., Yang, P., Xue, J., Chen, C., Sun, Q., et al., (2018). Involvement of HIF-1α-regulated miR-21, acting via the Akt/NF-κB pathway, in malignant transformation of HBE cells induced by cigarette smoke extract, Toxicology Letters, 289:14-21, https://doi.org/10.1016/j.toxlet.2018.02.027. https://www.sciencedirect.com/science/article/pii/S0378427418300730

(Miyashita, et al., 2022) Miyashita, Y., Yoshida, T., Takagi, Y. et al. Circulating extracellular vesicle microRNAs associated with adverse reactions, proinflammatory cytokine, and antibody production after COVID-19 vaccination. npj Vaccines 7, 16 (2022). https://doi.org/10.1038/s41541-022-00439-3 https://www.nature.com/articles/s41541-022-00439-3

(Nance, Meier, 2021) Nance, K.D., Meier, J.L., Modifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines. ACS Cent Sci. 2021 May 26;7(5):748-756. doi: 10.1021/acscentsci.1c00197. Epub 2021 Apr 6. PMID: 34075344; PMCID: PMC8043204. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043204/

(O’Brien, Hayder, Zayed, Peng, 2018) O'Brien, J., Hayder, H., Zayed, Y., Peng, C., (2018). Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation, Frontiers in Endocrinology, Vol. 9, DOI=10.3389/fendo.2018.00402 https://www.frontiersin.org/articles/10.3389/fendo.2018.00402

(Pang, et al., 2022) Pang, A.P.S., Higgins-Chen, A.T., Comite, F., Raica, I., Arboleda, C., Went, H., et al.,, (2022). Longitudinal Study of DNA Methylation and Epigenetic Clocks Prior to and Following Test-Confirmed COVID-19 and mRNA Vaccination, Frontiers in Genetics, Vol. 13, DOI=10.3389/fgene.2022.819749, https://www.frontiersin.org/articles/10.3389/fgene.2022.819749/full

“Complementary longitudinal epigenetic clock analyses of 36 participants prior to and following Pfizer and Moderna mRNA-based COVID-19 vaccination revealed that vaccination significantly reduced principal component-based Horvath epigenetic clock estimates in people over 50 by an average of 3.91 years for those who received Moderna.” (Pang, et al., 2022)

(Papagiannakopoulos, Shapiro, Kosik, 2008) Papagiannakopoulos, T., Shapiro, A., Kosik, K.S., (2008). MicroRNA-21 Targets a Network of Key Tumor-Suppressive Pathways in Glioblastoma Cells. Cancer Res 1 October 2008; 68 (19): 8164–8172. https://doi.org/10.1158/0008-5472.CAN-08-1305 https://aacrjournals.org/cancerres/article/68/19/8164/541735/MicroRNA-21-Targets-a-Network-of-Key-Tumor

(Paroo, Ye, Chen, Liu, 2009) Paroo Z, Ye X, Chen S, Liu Q. Phosphorylation of the human microRNA-generating complex mediates MAPK/Erk signaling. Cell. 2009 Oct 2;139(1):112-22. doi: 10.1016/j.cell.2009.06.044. PMID: 19804757; PMCID: PMC2760040. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760040/

“Taken together, these studies suggest that the MAPK/Erk signaling pathway regulates the miRNA machinery and suggests a general principle in which signaling systems target the miRNA signaling pathway to achieve biological responses." (Paroo, Ye, Chen, Liu, 2009)

***Which suggests why pomegranate peel or other polyphenols help as they modulate or inhibit MAPK activation. (Rasheed, Akhtar, Haqi, 2010)

(Patterson, et al., 2022, preprint) SARS-CoV-2 S1 Protein Persistence in SARS-CoV-2 Negative Post-Vaccination Individuals with Long COVID/ PASC-Like Symptoms 206f2ad5-a550-4359-a37e-18170828137a.pdf (researchsquare.com)

(Ponsuksili, et al., 2021) Ponsuksili, S., Oster, M., Reyer, H., Hadlich, F., Trakooljul, N., Rodehutscord, M., et al., (2021). Genetic regulation and heritability of miRNA and mRNA expression link to phosphorus utilization and gut microbiome. Open Biol. 2021 Feb;11(2):200182. doi: 10.1098/rsob.200182. Epub 2021 Feb 17. PMID: 33593158; PMCID: PMC8061690. https://royalsocietypublishing.org/doi/10.1098/rsob.200182

(Rasheed, Akhtar, Haqi, 2010) Rasheed, Z., Akhtar, N. & Haqqi, T.M. Pomegranate extract inhibits the interleukin-1β-induced activation of MKK-3, p38α-MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes. Arthritis Res Ther 12, R195 (2010). https://doi.org/10.1186/ar3166 https://arthritis-research.biomedcentral.com/articles/10.1186/ar3166

(Sass, et al., 2011) Sass, S., Dietmann, S., Burk, U.C., et al., (2011). MicroRNAs coordinately regulate protein complexes. BMC Syst Biol, 5, 136. https://doi.org/10.1186/1752-0509-5-136 https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-5-136

(Sattar, et al., 2023) Sattar, S., Kabat, J., Jerome, K., Feldmann, F., Bailey, K., Mehedi M., (2023). Nuclear translocation of spike mRNA and protein is a novel feature of SARS-CoV-2, Frontiers in Microbiology, Vol.14, DOI=10.3389/fmicb.2023.1073789, https://www.frontiersin.org/articles/10.3389/fmicb.2023.1073789

(Schoof, et al., 2015) Schoof, C.L.G., Izzotti, A., Jasiulionis, M.G., dos Reis Vasques, L., (2015). The Roles of miR-26, miR-29, and miR-203 in the Silencing of the Epigenetic Machinery during Melanocyte Transformation, BioMed Research International, vol. 2015, Article ID 634749, 11 pages, 2015. https://doi.org/10.1155/2015/634749 https://www.hindawi.com/journals/bmri/2015/634749/

"Taken together, these data suggest that epigenetic changes that occur early during malignant transformation may be due to the modulation of miR-26, miR-29, and miR-203 and the resulting effects on key genes involved in the epigenetic machinery." (Schoof, et al., 2015)

(Taki, Pan, Lee, Zhang, 2014) Taki FA, Pan X, Lee MH, Zhang B. Nicotine exposure and transgenerational impact: a prospective study on small regulatory microRNAs. Sci Rep. 2014 Dec 17;4:7513. doi: 10.1038/srep07513. PMID: 25515333; PMCID: PMC4894410. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894410/

(Uray, Major, Lontay, 2020) Uray K, Major E, Lontay B. MicroRNA Regulatory Pathways in the Control of the Actin-Myosin Cytoskeleton. Cells. 2020 Jul 9;9(7):1649. doi: 10.3390/cells9071649. PMID: 32660059; PMCID: PMC7408560. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7408560/

(Visser, Thomas, 2021) Visser, H., Thomas, A.D., (2021). MicroRNAs and the DNA damage response: How is cell fate determined?, DNA Repair, Vol. 108, 2021, 103245, ISSN 1568-7864, https://doi.org/10.1016/j.dnarep.2021.103245. https://www.sciencedirect.com/science/article/pii/S1568786421002019

(Xu, Gu, Thumati, Wong, 2017) Xu, J., Gu, A.Y., Thumati, N.R., Wong, J.M.Y., Quantification of Pseudouridine Levels in Cellular RNA Pools with a Modified HPLC-UV Assay. Genes (Basel). 2017 Sep 5;8(9):219. doi: 10.3390/genes8090219. PMID: 28872587; PMCID: PMC5615352. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615352/