Mg-ATP and cytoplasm; salt and salicylates; and aether energy of cosmic and local space.
Piccardi is mentioned by Pollack, 2013, and DeMeo, 2019, had a much fuller discussion of Piccardi's findings. *Post too long for email and common sense - but it is short compared to some of my posts.
Magnesium forms a 2+ ion which bonds with ATP, adenosinetriphosphate, at either 2 or 3 of its negatively charged phosphorus atoms.
“Hydrolysis of adenosine triphosphate (ATP) serves as a source of chemical energy across all domains of life, driving essentially all energy-consuming cellular processes. Both unbound in cellular surroundings and as enzyme substrates, nucleotide triphosphates occur predominantly with divalent cations, most commonly Mg2+, (1,2) coordinated with the negatively charged triphosphate moiety.”
Mitochondria are major storehouses for intracellular magnesium. A gene MRS2 encodes a transporter protein that imports magnesium across the mitochondrial inner membrane. Dysregulation of magnesium transport by knocking out the MRS2 gene led to disrupted ATP production due to a “shift of mitochondrial energy metabolism and morphology.” (Yamanaka, et a., 2016)
Abstract: “Cellular energy production processes are composed of many Mg2+ dependent enzymatic reactions. In fact, dysregulation of Mg2+ homeostasis is involved in various cellular malfunctions and diseases. Recently, mitochondria, energy-producing organelles, have been known as major intracellular Mg2+ stores. Several biological stimuli alter mitochondrial Mg2+ concentration by intracellular redistribution. However, in living cells, whether mitochondrial Mg2+ alteration affect cellular energy metabolism remains unclear. Mg2+ transporter of mitochondrial inner membrane MRS2 is an essential component of mitochondrial Mg2+ uptake system. Here, we comprehensively analyzed intracellular Mg2+ levels and energy metabolism in Mrs2 knockdown (KD) cells using fluorescence imaging and metabolome analysis. Dysregulation of mitochondrial Mg2+ homeostasis disrupted ATP production via shift of mitochondrial energy metabolism and morphology. Moreover, Mrs2 KD sensitized cellular tolerance against cellular stress. These results indicate regulation of mitochondrial Mg2+ via MRS2 critically decides cellular energy status and cell vulnerability via regulation of mitochondrial Mg2+ level in response to physiological stimuli.” (Yamanaka, et a., 2016)
The MRS2 gene encodes an enzyme that is primarily for transport of magnesium into mitochondria but also is involved in lactate metabolism. Diseases associated with dysfunction of the gene include cerebral vascular stroke or occlusion risks, see excerpt below. (genecards.org) We need adequacy of magnesium to prevent inflammation and calcification and for that we need adequacy of protein, folate and phospholipids to create ATP or other mineral transport proteins, as magnesium isn’t running around free within the body. Magnesium’s electrical activity must be contained with a protein mineral carrier or adenosine triphosphate. See: To have adequate magnesium, we need adequate protein and phospholipids too. (Substack)
MRS2 Gene - Magnesium Transporter MRS2
Protein Coding (Updated: Aug 2, 2023) (genecards.org)
GeneCards Summary for MRS2 Gene
MRS2 (Magnesium Transporter MRS2) is a Protein Coding gene.
Diseases associated with MRS2 include Middle Cerebral Artery Infarction and Basilar Artery Occlusion.
Among its related pathways are Transport of inorganic cations/anions and amino acids/oligopeptides and Miscellaneous transport and binding events.
UniProtKB/Swiss-Prot Summary for MRS2 Gene
Magnesium transporter that mediates the influx of magnesium into the mitochondrial matrix (PubMed:11401429, 18384665). Required for normal expression of the mitochondrial respiratory complex I subunits (PubMed:18384665). ( MRS2_HUMAN,Q9HD23 )
*The following is a different section of the same page about the MRS2 gene (genecards.org) - the GO…..link is a database of information about the mentioned topic. I found it interesting as excess lactic acid buildup can occur with methylation cycle dysfunction - a gene allele in this MRS2 gene could lead to low magnesium levels in mitochondria > decreased ATP production with a shift to anaerobic glycolysis from aerobic Citric Acid Cycle and that increases risk of lactic acid accumulation.
GO:0006089 3 involved_in lactate metabolic process Evidence: IEA
GO:0006811 5 monoatomic ion transport Evidence: IEA
GO:0015693 5 magnesium ion transport Evidence: IBA
GO:0045016 3 5 involved_in mitochondrial magnesium ion transmembrane transport Evidence: IMP, IBA Pubmed: 11401429
GO:0055085 3 5 involved_in transmembrane transport Evidence: TAS
Prevent obesity, with magnesium adequacy.
We not only have magnesium-ATP, we have magnesium-ATPase (Mg-ATPase) to break it apart AND we have calcium-ATP and calcium-ATPase (Ca-ATPase). And increased BMI was correlated with decreased Ca-ATPase in a study with obese women as participants. Mg-ATPase was positively correlated with Ca-ATPase. (Nassar, Hashim, Lachance, 2004) Addition - that suggests then decreased Mg-ATPase would be seen along with decreased Ca-ATPase in the participants with higher BMIs, I am not sure if that suggests low magnesium levels though. Increased calcium intracellularly is suggested by the authors to be a potential concern.
“Discussion: Ca-ATPase activity decreases as BMI increases. Decreased ATPase activity may contribute to increased intracellular calcium, previously reported in obese persons.” (Nassar, Hashim, Lachance, 2004)
Increased intracellular calcium increases inflammatory endocannabinoid release from membranes and would also condense cellular matrix proteins and dehydrate the gel like intracellular matrix more than might be desirable for optimal mitochondrial function and pH levels. Addition - Low magnesium would increase risk of overweight issues and hypertension, Metabolic Syndrome or Type 2 diabetes.
“Briefly, as an integral part of Mg-ATP, Mg is required for glycolysis and regulates all enzymes involved in phosphorylation, including phosphorylation of tyrosine kinase on the insulin receptor. It is also required for ATP and phosphate transfer enzymes that regulate ion transport such as Ca-ATPases in the plasma membrane, endoplasmic and sarcoplasmic reticulum. Thus, reduced intracellular Mg can reduce insulin signaling via tyrosine kinase, impair insulin action, and worsen insulin resistance. In fact, Mg deficit has been proposed as a common underlying mechanism of “insulin resistance” seen in metabolic syndrome, obesity, and other metabolic conditions.” (Ettinger, 2017)
Type II Diabetes, Peripheral Neuropathy, and Gout, Susan Ettinger, in Nutritional Pathophysiology of Obesity and its Comorbidities, 2017 viewable at (ScienceDirect)
From the Gerald H. Pollack Fourth Phase of Water book it is clear why sodium or calcium in excess in the wrong areas can cause big problems and quick shifts in fluid — they both affect the exclusion zones, causing condensing of the layers of structured water that form the gel like matrix of our intracellular and extracellular fluid — during health. Add a congesting situation and the water somehow turns into puffy body and a congested nose.
Prevent aging with magnesium adequacy too, by reducing ROS - reactive oxidative stress chemicals.
Magnesium helped reduced aging damage in a genetic mouse model of progeria - premature aging in childhood. (Villa-Bellosta, 2020)
Abstract: “Aging is associated with redox imbalance according to the redox theory of aging. Consistently, a mouse model of premature aging (LmnaG609G/+) showed an increased level of mitochondrial reactive oxygen species (ROS) and a reduced basal antioxidant capacity, including loss of the NADPH-coupled glutathione redox system. LmnaG609G/+ mice also exhibited reduced mitochondrial ATP synthesis secondary to ROS-induced mitochondrial dysfunction. Treatment of LmnaG609G/+ vascular smooth muscle cells with magnesium-enriched medium improved the intracellular ATP level, enhanced the antioxidant capacity, and thereby reduced mitochondrial ROS production. Moreover, treatment of LmnaG609G/+ mice with dietary magnesium improved the proton pumps (complexes I, III, and IV), stimulated extramitochondrial NADH oxidation and enhanced the coupled mitochondrial membrane potential, and thereby increased H+-coupled mitochondrial NADPH and ATP synthesis, which is necessary for cellular energy supply and survival.
»Consistently, magnesium treatment reduced calcification of vascular smooth muscle cells in vitro and in vivo, and improved the longevity of mice. This antioxidant property of magnesium may be beneficial in children with HGPS [Hutchinson-Gilford Progeria Syndrome].” (Villa-Bellosta, 2020)
Cholesterol is not the culprit in calcification of soft tissue - it is more like spackle to try to seal over a dangerous metal from being electrically active in the wrong places of the body. Reducing cholesterol levels with statins is just removing a safety tool the body is using to try to control calcium when there is too little magnesium available to keep ion channels closed.
Gerald H. Pollack makes it clear that he is a BIG fan of his exclusion zone theory (The Fourth Phase of Water book), possibly to the exclusion of recognizing that membranes and ion channels also have a role to play in health and mitochondrial function too. Health is complex. Exclusion zone theory is not wrong in my opinion, that is not my point, but that doesn’t make TRP and other ion channels a non-essential part of health. Producing a ‘theory’ tends to require simplifying an issue enough to see the underlying points, but the theory still needs to fit into the larger whole too and TRP and other ion channels are ancient - across species - they have a role in healthy cytoplasm too, in my opinion. Why else would hot pepper make our noses run and eyes water? Addition: They are activating TRP channels and increasing flow of thinner mucus by changing the calcium or chloride concentration inside of cells. More calcium would condense proteins and force fluid out of the exclusion zones within the intracellular matrix.
When two books by an author on a topic related to magnesium and iodine do not include much or any mention of magnesium or iodine - it makes me suspicious of their review of background literature - or their goals. The insider cult loves Einstein and loves not talking about aether energy and loves not talking about magnesium and iodine in health. Pollack is very complimentary of Einstein, but is also disagreeing with Einstein’s theory about Brownian motion - so maybe he is just scientifically polite about someone who is idolized.
*Just a observation of mine. Patterns are worth recognizing and thinking about, but may also be coincidental. Reminder - Einstein was a 26 year old patent clerk with a brilliant older wife who he later cheated on, deserting her and their two children. He may have been a little ADHD/autistic and apparently she helped him write the research papers. And at a divorce when she demanded the Nobel Prize money he had recently been given → he gave it to her → suggesting he thought she did deserve the award even though he hadn’t included her name as Maria Curie’s husband had insisted on.
Einstein ‘brilliant’ theories were also so similar to another scientist’s theory at the time that it was initially always referred to with both of their names, not just Einstein (from the DeMeo book, Reich and Einstein had met and Reich gave him one of his aether collecting devices to test but Einstein never publicly confirmed Reich’s work was accurate, or debunked it as wrong either - the insider cult ignores or suppresses any information that they don’t want the public or academia to explore further).
Einstein’s ‘brilliant’ papers are all math theory and direct observations in many ways refute or find anomalies with the ‘space is empty’ part of his work, which the rest of it kind of depends on being true. E may still = MC squared, but it also needs to be multiplied by a variable for the local speed of aether flow - which varies with altitude, season, and weather conditions.
Reminder » Do not worship false idols, whether they be celebrities in Hollywood, science, or a religious like group - or set yourself up on your own pedestal either - we all have an Achilles Heel. (puntentional - we do all have Achilles tendons in our foot and they may be a weak spot as it was said to be for Achilles in Greek mythology. (versions of the story vary: interestingliterature.com)
Personal diet/autoimmune issues - salt and salicylate and adrenal fatigue/overwork from stress.
With my own recent diet issues, avoiding salicylates has helped reduce the puffy leg edema but avoiding salt fairly stringently seems just as important and avoiding emotional stress. I also seem to need diuretics daily - pomegranate peel, sumac and or dandelion tea can all help. Those can be salicylate sources too, so moderation is important. The salicylate issue is helped by my taking dimethylglycine daily and avoiding becoming overly acidic which the high dose niacin protocol tends to push me. Addition - See my page on Salicylate sensitivity for more info: (Substack).
A little salt in the morning helps restore balance after a diuretic night but then I try not to use much salt for the rest of the day - except I love it. Craving salt tends to go along with adrenal insufficiency which can be a cause of puffy leg edema but so can congestive heart failure and other heart or kidney issues - so self diagnosing can be a dumb thing to do too. I seem to have some kidney and heart issues. My genetics makes excess homocysteine a long term risk of which I have had symptoms - more obvious in childhood when I had to follow my mother’s diet demands. I hated milk and did have PICA when young - I often gnawed on candle wax or crayons as an example. I think I had a phospholipid deficiency. I ate geranium leaves as a toddler and geraniums are bitter - I can’t quite imagine eating them on purpose.
I likely need more calcium but with enough vitamin K2 to keep it bone matrix instead of adding to uncomfortable bone spurs or calcinosis tissue deposits. My ‘vegan’ diet has been a lot more satisfying with the addition of sardines. ;-)
There are 32-34 micrograms of vitamin A in sardines, and zero in tilapia fillets - which is good as a cold fish salad. My mom enjoyed her tilapia salad with a spoon of mayo and a spoon of pure maple syrup. Addition - see my page on Retinoid Toxicity for why I consider vitamin A or carotenoid content of foods in my diet. (Substack)
And there is 569 mg calcium in 149 gram serving of sardines (nutritionvalue.org/Sardines_canned_in_oil) Maybe I really need to pay more attention to my calcium intake - which would help balance phosphorus excess which is part of the over-acidity and kidney load problem too. Addition - post about phosphorus content in foods. (Substack) Excess in ratio to calcium leads to demineralization of bone matrix and tooth enamel.
Worth noting: Health looks easy until you don’t have it.
A useful Substack warning about “sepsis” being the new code word for immune dysfunction following CoV jabs.
The intravenous vitamin C, thiamine and corticosteroid protocol by Marik would help. Low sodium is mentioned as a risk factor - we do need sodium in balance and mainstream medical does tend to demonize salt as if each and every salt granule is the reason for heart or blood pressure issues (instead of low magnesium or other reasons). Salt excess can be easy to do with modern foods though too. Processed or restaurant foods tend to be high sodium and that will be a negative factor for our cellular matrix/cytoplasm.
A Giorgio Piccardi aside -
The Pollack Fourth Phase of Water book discusses research by Giorgio Piccardi whose research is also included by James DeMeo in his 2019 book Correcting a Major Error in Modern Science The Dynamic Ether of Cosmic Space (pp 229-230, 251-254, quote from 257) (Amazon.com).
“The work of Giorgio Piccardi, discussed below, also found similar cosmic effects in phase-change and chemical reactions in water, occurring simultaneously in both northern and southern hemispheres. It was not something due only to seasonal temperature variations, or related mechanical findings.” (DeMeo, 2019, p257) Image below, pages 258-259.
Aside, we are moving through cosmic space in a large loose spiral along with everything else and we all heading towards/beyond the Vega star. * That is from an earlier book summary series I haven't finished, we are now colliding or condensing book review topics. See this post for links to the series - I didn’t get back to it after this grab-bag of a post: Air flow, aether, and Van Gogh's Starry Night, Chapter Four continued; & Lion’s Mane mushroom and Avocado seed flour - two super foods. / Link to Vejon Covid-19 Review, re autoimmune risk. (Substack)
The information gathered by Pollack is supportive of there being some type of energy connected with cosmic space time signatures and which is attracted to water and to tubes, like capillary blood vessels. Gerald H. Pollack moves on from Piccardi’s findings leaving it a mystery and focuses on the flow of H+ proton ions attracted to negative surfaces or areas of exclusion zones. Water can act like a battery and separate into positive and negative zones with more hydroxyl groups OH- and positive zones with more H+. pH sensitive dye in the water will show different areas of more H+ acidity or more OH- alkalinity.
Aether energy is neither electricity or magnetism as typically understood but it is attracted to flowing water and its peaks and ebbs in velocity of flow matches the seasonal changes measured by Giorigo Piccardi. Aether energy is everywhere, but less flow is measured underground or near sea level than up in airy mountainous regions. an enclosed concrete basement would have little measurable flow of the cosmic wind a somewhat colorful but kind of descriptive phrase for aether energy.
Is it just hydrogen protons being attracted to negative proteins or water hydroxyl groups? I don’t think so because the exclusion zones are in other solvents besides water too and water like flow in outer space spiral nebula clouds look a lot like watery spirals in a river as you paddle a canoe.
Aether energy has to do with the aura of the glowing ion in the Northern Lights and indigenous people there have some fear and respect for the lights as there are tales of the aura light reaching down for people - is god nature? Is cosmic energy nature? is cosmic energy god? Are we all cosmic energy and all part of nature god? Is water god? Is there Living Water? Is Living Water structured water full of aether energy? Also known as chi? That’s me speculating.
Disclaimer: This information is being provided for educational or entertainment purposes within the guidelines of Fair Use and is not intended to provide individual health guidance.
Reference List
Adenosine Triphosphate Magnesium, ScienceDirect.com, https://www.sciencedirect.com/topics/neuroscience/adenosine-triphosphate-magnesium, quoted: Type II Diabetes, Peripheral Neuropathy, and Gout, Susan Ettinger, in Nutritional Pathophysiology of Obesity and its Comorbidities, 2017.
(Buelens, et al., 2021) Buelens, F.P., Leonov, H., de Groot, B.L., Grubmüller, H., (2021), ATP–Magnesium Coordination: Protein Structure-Based Force Field Evaluation and Corrections, J of Chemical Theory and Computation, 17(3);1922-1930, DOI: 10.1021/acs.jctc.0c01205 https://pubs.acs.org/doi/10.1021/acs.jctc.0c01205
(Nassar, Hashim, Lachance, 2004) Nasser, J.A., Hashim, S.A., Lachance, P.A., (2004), Calcium and magnesium ATPase activities in women with varying BMIs. Obes Res. Nov;12(11):1844-50. doi: 10.1038/oby.2004.229. PMID: 15601981. https://onlinelibrary.wiley.com/doi/10.1038/oby.2004.229
(Villa-Bellosta, 2020) Villa-Bellosta, R., (2020), Dietary magnesium supplementation improves lifespan in a mouse model of progeria, EMBO Mol Med 12: e12423 https://doi.org/10.15252/emmm.202012423 https://www.embopress.org/doi/full/10.15252/emmm.202012423
(Yamanaka, et a., 2016) Yamanaka, R., Tabata, S., Shindo, Y., Hotta, K., Suzuki, K., Soga, T., Oka, K., (22016), Mitochondrial Mg(2+) homeostasis decides cellular energy metabolism and vulnerability to stress. Sci Rep. 2016 Jul 26;6:30027. doi: 10.1038/srep30027. https://www.nature.com/articles/srep30027 PMID: 27458051; PMCID: PMC4960558. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960558/
I scanned this because I really have to go outside right now, but was so excited to see all this information I could not resist. seriously thinking about taking that speed reading class just to keep up with you! and that is just so I can read your posts, not even thinking about reading all the references you have gone through! signed, huge fan.